Chromatography Workbook

MSL975066

Headspace GC Alcohol — Peak AREA

Use Area from the GC printout, not Height.

Standards

StandardAlcohol added (µL)Ethanol concentration %v/vEthanol peak areaPropanol peak areaEthanol/Propanol peak ratio

Calibration graph

Samples — two entries

SampleDilution factorEthanol peak areaPropanol peak areaPeak ratioConcentration from calibration %v/vFinal ethanol %v/vFinal ethanol %w/v
Sample calculation: peak ratio → concentration from calibration → × dilution factor → final %v/v → × 0.7894 → final %w/v. The graph uses the same calculated concentration value for the sample drop line.

HPLC Sugar

Enter peak areas. Separate calibration graphs are provided for fructose, glucose and sucrose.

Ion Chromatography

Enter standard concentration and peak area. Calibration graph is generated from your entries.

Headspace GC Alcohol — Good Results — Peak AREA

Use Area from the GC printout, not Height. Data below are entered from the Headspace sheet in Lims - Sample(4).xlsx.

Standards

StandardAlcohol added (µL)Ethanol concentration %v/vEthanol peak areaPropanol peak areaEthanol/Propanol peak ratio

Calibration graph

Samples — two entries

SampleDilution factorEthanol peak areaPropanol peak areaPeak ratioConcentration from calibration %v/vFinal ethanol %v/vFinal ethanol %w/v
Sample calculation: peak ratio → concentration from calibration → × dilution factor → final %v/v → × 0.7894 → final %w/v. The graph uses the same calculated concentration value for the sample drop line.